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Journal: Frontiers in Immunology
Article Title: Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survival
doi: 10.3389/fimmu.2025.1617146
Figure Lengend Snippet: Identification of OC macrophages during the chronic phase of cyclodextrin ototoxicity. (A) A side view of the sensory epithelium stained for CD45 and Iba1 from a cochlea examined 6 weeks post-treatment. The two downward arrows at the top indicate OC macrophages, and the upward arrow at the bottom indicates the BM macrophage. The dark space marked by the asterisks is the basement membrane, which separates the OC macrophages from the BM macrophages. (B) Top view of the sensory epithelium displaying OC macrophages. This image was created by removing the confocal image layers containing BM macrophages from a 3D stack, thus revealing only OC macrophages. Arrows indicate OC macrophages with different morphologies. (C) Quantification of OC macrophages at different regions along the cochlear spirals. (* indicates p < 0.05, ** indicates p < 0.01) (D) A schematic of the cochlear spiral illustrates the distribution of OC macrophages. OC macrophages are primarily confined to the basal region (70–90% from the apex). In contrast, hair cell loss extends across the entire basal and middle regions of the cochlea. (E) OC macrophages are present in the cochlea 2 weeks post-treatment (arrows). (F) A cross-sectional view of the sensory epithelium confirms that these macrophages are located within the organ of Corti (downward arrows). The asterisks indicate the basement membrane that separates the OC macrophages (downward arrows) from the BM macrophage (upward arrow). ns, not significant; OC, organ of Corti.
Article Snippet: This distinction was made using cross-sectional views of
Techniques: Staining, Membrane